Prabha Bisht , Manu Pant , J. M. S. Chauhan

Abstract

The present study involves in-vitro clonal propagation of Tylophora indica (Burm.f.) Merrill through axillary bud sprouting and subsequent plant regeneration. Axillary shoot proliferation was obtained using nodal segments as explants. Cultures were established on MS medium supplemented with BAP and Kn (0.5·3.0mg/l) individually and in combination with NAA (0.1 and 0.5 mg/l) and regular sub-culturing was carried out in BAP (1.0 mg/l) at periodic intervals of 25 days. Maximum number of shoots developed after 30 and 60 days was registered as 2.63±0.23 and 4.63±0.26, in MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. Best ischolar_maining was observed in ½ strength MS medium having 0.5 mg/l IBA with maximum number of ischolar_mains per shoot 2.18±0.22 in 30 days and 3.27±0.42 in 60 days with ischolar_main length 0.62±0.07cm and 8.22±0.15cm in30 and 60 days respectively. The plantlets were successfully hardened and transferred to earthen pots.

Keywords

Clonal Propagation, Axillary Bud, Tylophora indica, Multipurpose, Multiplication,
Shoot Initiation

Full Text

     

V. K. Sharma , Parveen , D. P. Uniyal , Prabha Bisht

Abstract

No abstract

Full Text

     

V. K. Sharma , Parveen , D. P. Uniyal , Prabha Bisht

Abstract

No abstract

Full Text

     

V. K. Sharma , Ila Joshi , Prabha Bisht , S. K. Bagchi

Abstract

No abstract

Full Text

     

Manu Pant , Prabha Bisht

Abstract

A procedure for the regeneration of complete plantlets of Tylophora indica from cultured leaf callus is described. Callus was obtained from leaf explants on MS medium supplemented with varying concentrations and combinations of BAP, 2,4-D and TDZ. 2,4-D (1.35μM) in combination with TOZ (0.68 μM) proved to be most effective for callus induction. Adventitious shoots were regenerated from surface of callus after transferring onto shoot induction medium, the optimal hormone combination being BAP (8.88 μM),IAA (1.14 μM) and GA, (0.58 μM) giving an average of 14.25 ± 0.62 shoots with mean shoot length 1.9 ± 0.06 cm after 4 weeks. Individual elongated shoots were ischolar_mained on half-strength MS medium containing 2.46μM IBA. The in-vitro raised plantlets with well developed shoot and ischolar_mains were acclimatized successfully and shifted to field conditions with a survival rate of over 90%.

Keywords

Tylophora Indica, In-vitro Regeneration, Leaf Explant, Callus Culture

Full Text

     

J. M. S. Chauhan ¹, Prabha Bisht ¹, Monika Panwar ¹, Ajay Thakur ¹

Affiliations
1 Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun (Uttarakhand), IN

Abstract

The present study involves in-vitro clonal propagation of Elaeocarpus sphaericus through axillary bud sprouting and subsequent shoot regeneration. Optimal proliferations of axillary shoot were induced on MS medium supplemented with BAP (1.5 mg/l) and KN (1.5 mg/l). Optimal shoot multiplication was induced on MS medium fortified with BAP (1.5mg/l), KN (1.5 mg/l) and CH (100 mg/l). MS medium supplemented with 1.0 mg/l BAP and 50 mg/l ADS was used for routine sub-culturing of multiple shoots at periodic interval of every 3 weeks. Of the 6 treatments (3 each of IBA and NAA) imposed for ischolar_maining of shoots, 1/2 MS + 1.0 mg/l IBA induced 80% ischolar_maining. Plants were hardened in vitro and acclimatized in hardening chamber and subsequently transferred to field.

Keywords

Elaeocarpus Sphaericus, In-Vitro Propagation, Nodal Explant, Axillary Shoot Sprouting.

Full Text